By Walter G. Berl
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The extinction of fluorescence is much more pronounced after previous spraying with fluorescing compounds (fluoresceine, acridine). A filtered mercury lamp (255 πΐμ) is a convenient light source. The UV absorption can be measured along the paper strip by analysis in a spectrophotometer. Infrared absorption may be used for quantitative analysis, as for amino acids (44). Radioactive substances can be detected by a sensitive silver bromide film pressed against the strip (autoradiography), by activity measurement along the strip with a Geiger counter or by automatic counting of radioactivity (103).
27. 28. 29. 30. 31. 32. 33. 34. 35. COURTENAY S. G. PHILLIPS Keulemans, A. I. , Anal. Chim. Ada 13, 357 (1955). Lichtenfels, D. , Fleck, S. , and Burow, F. , Anal, Chem. 27, 1510 (1955). Littlewood, A. , Phillips, C. S. , and Price, D. , J. Chem, Soc. p. 1480 (1955). Martin, A. , Nature 175, 422 (1955). Martin, A. J. , and Synge, R. L. , Biochem. J. 35, 1358 (1941). Patton, H. , Lewis, J. , and Kaye, W. L, Anal. Chem. 27, 171 (1955). Phillips, C. S. , Discussions Faraday Soc. No. 7, 241 (1949).
Infrared absorption may be used for quantitative analysis, as for amino acids (44). Radioactive substances can be detected by a sensitive silver bromide film pressed against the strip (autoradiography), by activity measurement along the strip with a Geiger counter or by automatic counting of radioactivity (103). Radioactivity can be introduced by (a) use of radioactive mixtures, (b) subsequent reaction with radioactive substances and removal of excess reagent, (c) introduction of activity through neutron bombardment in sulfur-, bromine-, chlorine-, and phosphorus-containing substances (124, 125).