By Patrick J. Lincoln
Royal university of medication and Dentistry, London, united kingdom. state of the art advances in identification checking out via DNA research. Covers PCR-based attempt structures, direct-phase minisequencing, and extra. DNLM: DNA Fingerprinting--methods--laboratory manuals.
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Extra resources for Forensic DNA Profiling Protocols (Methods in Molecular Biology)
Example text
The 3X stain extraction buffer (5) is modified to meet these requirements; also, it does not contain dithiothreitol (DTT). It must be prewarmed before use. 5. In contrast to a previously published method(1), a step is omitted in this new protocol. This step involved cleaning of the outside of the tube with ethanol and punching a hole with a sterile needle in the bottom of the tube. The solution was then transferred to a new tube by the piggyback method via centrifugation at Page 31 1000g for 5 min.
Am. J. Human Genet. 48, 137 44. 16. , and Gill, P. (1994) Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci. Int. J. Legal Med. 106, 302. 17. Holland, M. , Fisher, D. , Roby, R. , and Weedn, V. W. Crime Lab. Dig. 22, 109 15. 18. , Del Rio, S. , Turner, K. , Marion, M. , Weaver, K. , and Williams, P. E. (1995) Automated DNA purification and amplification from blood-stained cards using a robotic workstation. Biotechniques 19, 426 32. 19.
Lincoln and J. Page 20 room temperature (SDS precipitates at 4 ). Add proteinase K fresh from frozen aliquots of 20 mg/mL. 2. 5 EDTA-Na . Store at 4 . 0, 1 mM 2 3. Water-saturated n-butanol. Prepare fresh before use. 4. Centricon 100 microconcentrator tubes (Amicon, Danvers, MA). 5. 0+ Human DNA Quantitation System (Gibco-BRL, Gaithersburg, MD). For recovery of sperm-cell and vaginal-cell DNA(1 ): 6. 5. Store at 4 . 7. 5. 2 2 4 4 8. Sarcosyl: 20 and 10%. Store at 4 . 9. 0. Store at 4 . 2 10. 39 M.