By Shaoguang Li, Haojian Zhang
This quantity highlights the molecular and mobile tools utilized in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem phone biology. Written within the hugely winning Methods in Molecular Biology series structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, with ease reproducible laboratory protocols, and tips about troubleshooting and warding off identified pitfalls.
Authoritative and state-of-the-art, Chronic Myeloid Leukemia: equipment and Protocols goals to make sure winning ends up in the additional examine of this important field.
Read or Download Chronic Myeloid Leukemia: Methods and Protocols PDF
Similar forensic medicine books
The Analysis of Controlled Substances (Analytical Techniques in the Sciences (AnTs) *)
Offering new advancements in sampling and drug profiling, this publication additionally offers useful details on tips to perform research, what the consequences suggest and the way they are often used as courtroom proof and for medicinal drugs intelligence reasons. * contains case-studies with complete facts and spectra, aiding readers to spot components * Accessibly prepared via classification of compound * comprises an updated record of the most recent medications
Commingled human remains : methods in recovery, analysis, and identification
Commingled Human continues to be: tools in restoration, research, and id brings jointly instruments from different resources in the forensic technology group to supply a suite of accomplished methods to resolving matters linked to commingled continues to be. This variation specializes in forensic events, even supposing a few examples from prehistoric contexts also are addressed.
Using The MMPI-2 in Forensic Assessment
The Minnesota Multiphasic character Inventory-2 (MMPI-2) is among the most generally researched and used evaluation instruments in psychology. Forensic psychologists frequently depend upon it to judge consumers excited by civil and legal circumstances. as the try out effects may have an important effect on courtroom judgements, psychologists have to know how to make complete use of the MMPI-2 in forensic settings.
In lacking and unidentified investigations, an abyss of dissonance turns out to exist among legislations enforcement and the group they serve that each one too usually creates grating wounds that can by no means heal. using Forensic applied sciences for Unidentified Human is still: dying research assets, options, and Disconnects bridges this abyss.
- The Tumor Microenvironment: Methods and Protocols
- The Sexual Exploitation of Children: A Practical Guide to Assessment, Investigation, and Intervention, Second Edition
- Time of Death, Decomposition and Identification: An Atlas
- Alkyl polyglucosides : from natural-origin surfactants to prospective delivery systems
- Investing in Strategies to Reverse the Global Incidence of Tb (UN Millennium Project)
- Crime Laboratory Management
Additional info for Chronic Myeloid Leukemia: Methods and Protocols
Sample text
7. Spin cells at 500 Â g for 5 min again, and remove the supernatant. 8. Resuspend the cell pellet in fluorescence-activated cell sorting (FACS) buffer, and adjust cell concentration to 1 Â 107 cells/mL. 9. Stain cells with fluorescence-labeled myeloid cell marker (Gr-1): add phycoerythrin (PE) or allophycocyanin (APC)-labeled Gr-1 antibody to 50 μL of cells. Mix well, and stain the cells at 4 C for 15–30 min. 10. Add 1 mL of PBS buffer to the cells, and wash cells once by spinning at 500 Â g, 4 C for 5 min.
3. Remove the NIH3T3 cell medium, and add premixed virus supernatant for infection. After 3 h culture, remove the virus supernatant, and change to 10 mL fresh medium. 4. After 48 h postinfection, digest cells with trypsin, and collect cells into 1 mL flow cytometry buffer. Take 300 μL cell suspension to run flow cytometry analysis for percentage of GFPþ cells. Normally, the good retroviral supernatant means the % GFP can reach to 90–95 % at the 1:2 dilution, 75–85 % at 1:8 dilution, and 60–70 % at 1:16 dilution.
7. Incubate cells in incubator at 37 C, 5 % CO2 for 3 h. Note: If the cells accumulate in the corner, gently shake or blow the clot to make it loose. 8. After 3 h incubation, gently remove supernatant with pipette to new tubes and centrifuge at 300 Â g for 10 min to return the removed cells; continually culture the cells with 4 ml fresh stimulation medium overnight. 2 ml, 100Â penicillin/strep, 40 μl, 100Â L-glutamine 40 μl, Ciproxin 4 μg, IL-3 4 μg, Il-6 4 μg, and SCF 4 μg. Note: You could make the culture medium half hour before changing medium.